CD107a / LAMP1 Monoclonal antibody, PBS Only

CD107a / LAMP1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B2E9

Cat no : 67300-1-PBS

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker

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Validation Data Gallery

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  WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

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  WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67300-1-Ig (same clone as 67300-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67300-1-Ig (same clone as 67300-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67300-1-Ig (same clone as 67300-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67300-1-Ig (same clone as 67300-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67300-1-Ig (same clone as 67300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67300-1-Ig (same clone as 67300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67300-1-PBS targets CD107a / LAMP1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD107a / LAMP1 fusion protein Ag28869
• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 417 aa, 45 kDa
• Observed Molecular Weight: 90-120 kDa
• GenBank Accession Number: BC021288
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.