TCP1 eta Polyclonal antibody

TCP1 eta Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15994-1-AP

Synonyms

CCT7, CCT ETA, HIV 1 Nef interacting protein, Chaperonin containing T-complex polypeptide 1 subunit 7, CCTH

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Validation Data Gallery

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  WB analysis of HeLa using 15994-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15994-1-AP (CCT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15994-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15994-1-AP (CCT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15994-1-AP

  IP result of anti-CCT7 (IP:15994-1-AP, 4ug; Detection:15994-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

• 

  IF Staining of MCF-7 using 15994-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CCT7 antibody 15994-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

15994-1-AP targets TCP1 eta in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TCP1 eta fusion protein Ag8868
• Full Name: chaperonin containing TCP1, subunit 7 (eta)
• Calculated Molecular Weight: 543 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC019296
• Gene Symbol: CCT7
• Gene ID (NCBI): 10574
• RRID: AB_2073903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.

Publications

Species	Application	Title
human	WB	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
	WB	Nat Commun; Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.; Authors - Adela S Oliva Chávez
human	IHC	Aging (Albany NY); Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma.; Authors - Xinghua Huang
mouse	WB	Virology; Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.; Authors - Sandhya Gopal
human	IHC	Medicine (Baltimore); Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study; Authors - Ruijuan Du
human	WB	Clin Proteomics; Quantitative proteomics identified circulating biomarkers in lung adenocarcinoma diagnosis; Authors - Hongyu Chen