CCT6A-Specific Polyclonal antibody

CCT6A-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19793-1-AP

Synonyms

CCT6A, Cctz, CCT6, CCT zeta 1, CCT zeta



Tested Applications

Positive WB detected inA431 cells, A549 cells. HeLa cells, mouse liver tissue, EC109 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19793-1-AP targets CCT6A-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name chaperonin containing TCP1, subunit 6A (zeta 1)
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_001762
Gene Symbol CCT6A
Gene ID (NCBI) 908
RRIDAB_10638922
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT6A, also named as CCT6, CCTZ, CCT-zeta,Tcp20 and HTR3, belongs to the TCP-1 chaperonin family. CCT6A is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. CCT6A is known to play a role, in vitro, in the folding of actin and tubulin. The antibody has no cross reaction to CCT6B.

Protocols

Product Specific Protocols
WB protocol for CCT6A-Specific antibody 19793-1-APDownload protocol
IHC protocol for CCT6A-Specific antibody 19793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell

CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.

Authors - Xinyu Ma
humanWB

Cancer Lett

Molecular chaperone CCT3 supports proper mitotic progression and cell proliferation in hepatocellular carcinoma cells.

Authors - Yuanyuan Zhang
humanWB

Mol Cell Proteomics

Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities.

Authors - Hussein Kaddour
humanWB,IHC

Oncol Lett

CCT6A is associated with CDC20, Enneking stage and prognosis in osteosarcoma, and its knockdown suppresses osteosarcoma cell viability and invasion

Authors - Ning Wang
mouseIF

Signal Transduct Target Ther

Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease

Authors - Boyuan Ma
humanWB,IF,IP,CoIP

J Transl Med

CCT6A facilitates lung adenocarcinoma progression and glycolysis via STAT1/HK2 axis

Authors - Shao-Kun Yu