CCT6A-Specific Polyclonal antibody

CCT6A-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 19793-1-AP

Synonyms

CCT6A, Cctz, CCT6, CCT zeta 1, CCT zeta

---

Validation Data Gallery

• 

  WB analysis using 19793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19793-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 19793-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 19793-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19793-1-AP (CCT6A-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A431 cells, A549 cells. HeLa cells, mouse liver tissue, EC109 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

19793-1-AP targets CCT6A-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: chaperonin containing TCP1, subunit 6A (zeta 1)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_001762
• Gene Symbol: CCT6A
• Gene ID (NCBI): 908
• RRID: AB_10638922
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT6A, also named as CCT6, CCTZ, CCT-zeta,Tcp20 and HTR3, belongs to the TCP-1 chaperonin family. CCT6A is a molecular chaperone which assists the folding of proteins upon ATP hydrolysis. CCT6A is known to play a role, in vitro, in the folding of actin and tubulin. The antibody has no cross reaction to CCT6B.

Publications

Species	Application	Title
human	WB,IF	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
human	WB	Cancer Lett; Molecular chaperone CCT3 supports proper mitotic progression and cell proliferation in hepatocellular carcinoma cells.; Authors - Yuanyuan Zhang
human	WB	Mol Cell Proteomics; Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities.; Authors - Hussein Kaddour
human	WB,IHC	Oncol Lett; CCT6A is associated with CDC20, Enneking stage and prognosis in osteosarcoma, and its knockdown suppresses osteosarcoma cell viability and invasion; Authors - Ning Wang
mouse	IF	Signal Transduct Target Ther; Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease; Authors - Boyuan Ma
human	WB,IF,IP,CoIP	J Transl Med; CCT6A facilitates lung adenocarcinoma progression and glycolysis via STAT1/HK2 axis; Authors - Shao-Kun Yu