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  • KD/KO Validated

CCT5 Polyclonal antibody

CCT5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11603-1-AP

Synonyms

CCT epsilon, CCT5, CCTE, KIAA0098, TCP 1 epsilon



Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, A431 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11603-1-AP targets CCT5 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCT5 fusion protein Ag2146
Full Name chaperonin containing TCP1, subunit 5 (epsilon)
Calculated molecular weight 541 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC035499
Gene symbol CCT5
Gene ID (NCBI) 22948
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.

Protocols

Product Specific Protocols
WB protocol for CCT5 antibody 11603-1-APDownload protocol
IHC protocol for CCT5 antibody 11603-1-APDownload protocol
IF protocol for CCT5 antibody 11603-1-APDownload protocol
IP protocol for CCT5 antibody 11603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.

Authors - Xinyu Ma
  • KD Validated
mouseWB

Nat Genet

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Authors - Tarkar Aarti A
humanWB,IF

Proc Natl Acad Sci U S A

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Authors - Seo Seongjin S
humanWB, IF

J Cell Biol

CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.

Authors - Yu-Cheng Zhang
chickenWB,IF

Front Microbiol

Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.

Authors - Xiaohan Zhang
  • KD Validated
humanWB,IF,CoIP

Front Med (Lausanne)

Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway.

Authors - Lujia Feng
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Andrea (Verified Customer) (01-03-2023)

Strong and clear band on the membrane.

  • Applications: Western Blot