CCT5 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCT5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 11603-1-AP

Synonyms

T-complex protein 1 subunit epsilon, KIAA0098, Chaperonin containing T-complex polypeptide 1 subunit 5, CCT-epsilon, CCTE

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Validation Data Gallery

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  WB analysis using 11603-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11603-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11603-1-AP

  WB result of CCT5 antibody (11603-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT5 transfected HEK-293 cells.

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  WB analysis of HeLa using 11603-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11603-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11603-1-AP (CCT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11603-1-AP

  IP result of anti-CCT5 (IP:11603-1-AP, 4ug; Detection:11603-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human colon cancer using 11603-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 11603-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 11603-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11603-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11603-1-AP (CCT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11603-1-AP

  Immunofluorescent analysis of HepG2 cells, using CCT5 antibody 11603-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells, A431 cells, HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• CoIP: See 2 publications below

Product Information

11603-1-AP targets CCT5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCT5 fusion protein Ag2146
• Full Name: chaperonin containing TCP1, subunit 5 (epsilon)
• Calculated Molecular Weight: 541 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC035499
• Gene Symbol: CCT5
• Gene ID (NCBI): 22948
• RRID: AB_2073774
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.

Publications

Species	Application	Title
human	WB	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma ; KD Validated
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A
human	WB,IF	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S
human	WB, IF	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
human,mouse	WB	Front Cell Dev Biol; TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis; Authors - Wen-Shuai Tang
chicken	WB,IF	Front Microbiol; Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.; Authors - Xiaohan Zhang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (01-03-2023)

Strong and clear band on the membrane.

• Applications: Western Blot