Validation Data Gallery
|Positive WB detected in||COLO 320 cells, HeLa cells, A431 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, mouse colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11603-1-AP targets CCT5 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CCT5 fusion protein Ag2146|
|Full Name||chaperonin containing TCP1, subunit 5 (epsilon)|
|Calculated molecular weight||541 aa, 60 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC035499|
|Gene ID (NCBI)||22948|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.
|Product Specific Protocols|
|WB protocol for CCT5 antibody 11603-1-AP||Download protocol|
|IHC protocol for CCT5 antibody 11603-1-AP||Download protocol|
|IF protocol for CCT5 antibody 11603-1-AP||Download protocol|
|IP protocol for CCT5 antibody 11603-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.
DYX1C1 is required for axonemal dynein assembly and ciliary motility.
Proc Natl Acad Sci U S A
BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.
J Cell Biol
CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.
Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins.
Front Med (Lausanne)
Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Andrea (Verified Customer) (01-03-2023)
Strong and clear band on the membrane.