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  • KD/KO Validated

CCT4 Polyclonal antibody

CCT4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21524-1-AP

Synonyms

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta



Tested Applications

Positive WB detected inMCF-7 cells, mouse heart tissue, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21524-1-AP is CCT4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCT4 fusion protein Ag16128
Full Name chaperonin containing TCP1, subunit 4 (delta)
Calculated Molecular Weight 539 aa, 58 kDa
Observed Molecular Weight 58-60 kDa
GenBank Accession NumberBC106934
Gene Symbol CCT4
Gene ID (NCBI) 10575
RRIDAB_10733520
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCT4 antibody 21524-1-APDownload protocol
IHC protocol for CCT4 antibody 21524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.

Authors - Xinyu Ma
  • KD Validated
humanWB

J Cell Biol

CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.

Authors - Yu-Cheng Zhang
humanWB

Mol Ther Nucleic Acids

hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4.

Authors - Qiang Luo
humanWB

Pathog Dis

Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.

Authors - Hirai Kimito K
humanWB

Mol Carcinog

CCT4 knockdown enhances the sensitivity of cisplatin by inhibiting glycolysis in human esophageal squamous cell carcinomas

Authors - Jiarui Fang
mouseWB

Sci Transl Med

Inhibiting de novo ceramide synthesis restores mitochondrial and protein homeostasis in muscle aging

Authors - Tanes I Lima