CCT4 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCT4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21524-1-AP

Synonyms

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta

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Validation Data Gallery

• 

  WB analysis of HeLa using 21524-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21524-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21524-1-AP (CCT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21524-1-AP (CCT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21524-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21524-1-AP (CCT4 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse heart tissue, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

The immunogen of 21524-1-AP is CCT4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCT4 fusion protein Ag16128
• Full Name: chaperonin containing TCP1, subunit 4 (delta)
• Calculated Molecular Weight: 539 aa, 58 kDa
• Observed Molecular Weight: 58-60 kDa
• GenBank Accession Number: BC106934
• Gene Symbol: CCT4
• Gene ID (NCBI): 10575
• RRID: AB_10733520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma ; KD Validated
human	WB	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
human	WB	Mol Ther Nucleic Acids; hsa_circ_0001018 promotes papillary thyroid cancer by facilitating cell survival, invasion, G1/S cell cycle progression, and repressing cell apoptosis via crosstalk with miR-338-3p and SOX4.; Authors - Qiang Luo
human	WB	Pathog Dis; Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.; Authors - Hirai Kimito K
human	WB	Mol Carcinog; CCT4 knockdown enhances the sensitivity of cisplatin by inhibiting glycolysis in human esophageal squamous cell carcinomas; Authors - Jiarui Fang
mouse	WB	Sci Transl Med; Inhibiting de novo ceramide synthesis restores mitochondrial and protein homeostasis in muscle aging; Authors - Tanes I Lima