CCPG1 Polyclonal antibody

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  WB analysis using 13861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13861-1-AP (CCPG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 13861-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13861-1-AP (CCPG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13861-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13861-1-AP (CCPG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13861-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCPG1 antibody (13861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CCPG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13861-1-AP

Publications(20)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KIAA1254, CPR8, CCP8

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, THP-1 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

13861-1-AP targets CCPG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCPG1 fusion protein Ag4841
• Full Name: cell cycle progression 1
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC034914
• Gene Symbol: CCPG1
• Gene ID (NCBI): 9236
• RRID: AB_2074010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULG6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell-cycle progression gene 1 (CCPG1) is a receptor for ER stress-induced ER-phagy. CCPG1 localizes to perinuclear ER and in small foci at the ER periphery. It contains a LIR at the N-terminal cytosolic domain engaging LC3/GABARAP. (PMID: 29802216, PMID: 29290589)

Publications

Species	Application	Title
human	WB	Nat Commun; AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity; Authors - Linliang Zhang
human	WB	Mol Cell; Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.; Authors - Susanne Zellner
human	WB	Mol Cell; Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.; Authors - Corey N Cunningham ; KD Validated
rat	WB	J Pineal Res; Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression; Authors - Min Yao
human	WB	Autophagy; ATF4 links ER stress with reticulophagy in glioblastoma cells.; Authors - Svenja Zielke
human	WB,IP	Dev Cell; CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.; Authors - Matthew D Smith ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (04-05-2020)

This antibody has lots of background bands. In the beginning, I was not sure which band is the right band. In the end, I performed knock-down experiment using siRNA, and validated that the band close to 98 kDa is the CCPG1 band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116

CCPG1 Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:13861-1-AP)