|Positive WB detected in||HepG2 cells, L02 cells, THP-1 cells|
|Positive IHC detected in||human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
13861-1-AP targets CCPG1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CCPG1 fusion protein Ag4841|
|Full Name||cell cycle progression 1|
|Calculated molecular weight||87 kDa|
|Observed molecular weight||110 kDa|
|GenBank accession number||BC034914|
|Gene ID (NCBI)||9236|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cell-cycle progression gene 1 (CCPG1) is a receptor for ER stress-induced ER-phagy. CCPG1 localizes to perinuclear ER and in small foci at the ER periphery. It contains a LIR at the N-terminal cytosolic domain engaging LC3/GABARAP. (PMID: 29802216, PMID: 29290589)
Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.
Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.
J Pineal Res
Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression
ATF4 links ER stress with reticulophagy in glioblastoma cells.
CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.
Mol Biol Cell
Lysosome Docking to WIPI1 Rings and ER-connected Phagophores Occurs During DNAJB12 and GABARAP-Dependent Selective Autophagy of Misfolded P23H-Rhodopsin.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
H (Verified Customer) (04-05-2020)
This antibody has lots of background bands. In the beginning, I was not sure which band is the right band. In the end, I performed knock-down experiment using siRNA, and validated that the band close to 98 kDa is the CCPG1 band.