Cyclin G Polyclonal antibody

Cyclin G Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10897-1-AP

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CCNG, CCNG1, CYCG1, Cyclin G, cyclin G1

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

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Filter: AllWBIHC

WB analysis of MDA-MB-453s using 10897-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse liver using 10897-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse ovary using 10897-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 10897-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 10897-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10897-1-AP (Cyclin G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10897-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10897-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10897-1-AP (Cyclin G antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (5)

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MSDS SDS

Tested Applications

Positive WB detected in	MDA-MB-453s cells, mouse ovary tissue, HeLa cells, mouse liver tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10897-1-AP targets Cyclin G in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1157 Product name: Recombinant human CCNG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-295 aa of BC007093 Sequence: MIEVLTTTDSQKLLHQLNALLEQESRCQPKVCGLRLIESAHDNGLRMTARLRDFEVKDLLSLTQFFGFDTETFSLAVNLLDRFLSKMKVQPKHLGCVGLSCFYLAVKSIEEERNVPLATDLIRISQYRFTVSDLMRMEKIVLEKVCWKVKATTAFQFLQLYYSLLQENLPLERRNSINFERLEAQLKACHCRIIFSKAKPSVLALSIIALEIQAQKCVELTEGIECLQKHSKINGRDLTFWQELVSKCLTEYSSNECSKPNVQKLKWIVSGRTARQLKHSYYRITHLPTIPEMVP Predict reactive species
Full Name	cyclin G1
Calculated Molecular Weight	34 kDa
Observed Molecular Weight	34 kDa
GenBank Accession Number	BC007093
Gene Symbol	Cyclin G
Gene ID (NCBI)	900
RRID	AB_2228613
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P51959
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Cyclin G Polyclonal antibody (Cat# 10897-1-AP) has 5 total citations. These appear in Cancer Letters, Oxidative Medicine and Cellular Longevity, Oncology Research, Journal of Biological Chemistry, and Experimental Cell Research. The associated research fields include pancreatic ductal adenocarcinoma, doxorubicin-induced cardiotoxicity, cholangiocarcinoma progression, hepatitis B virus replication, and colorectal cancer epigenetic regulation. All cited applications are Western blot in human or mouse samples.

Species	Application	Title
human	WB	Cancer Lett The C/EBPβ-LINC01133 axis promotes cell proliferation in pancreatic ductal adenocarcinoma through upregulation of CCNG1. Authors - Chen-Song Huang View Article
mouse	WB	Oxid Med Cell Longev miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1. Authors - Mingjing Yan View Article
human	WB	Oncol Res Calcyclin-binding protein contributes to cholangiocarcinoma progression by inhibiting ubiquitination of MCM2 Authors - Yusen Zhang View Article
human	WB	J Biol Chem MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1. Authors - Fanpeng Zhao View Article
human	WB	Exp Cell Res PRMT6 functionally associates with PRMT5 to promote colorectal cancer progression through epigenetically repressing the expression of CDKN2B and CCNG1 Authors - Yuzhong Chen View Article