CCDC53 Polyclonal antibody

CCDC53 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24445-1-AP

Synonyms

CCDC53, CGI 116, WASH complex subunit CCDC53, WASHC3, x0009

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Validation Data Gallery

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  IHC staining of human placenta using 24445-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 24445-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24445-1-AP (CCDC53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24445-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 24445-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 24445-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 24445-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24445-1-AP (CCDC53 Antibody) at dilution of 1:50.

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Tested Applications

• Positive IHC detected in: human placenta tissue, human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

24445-1-AP targets CCDC53 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC53 fusion protein Ag19831
• Full Name: coiled-coil domain containing 53
• Calculated Molecular Weight: 194 aa, 21 kDa
• GenBank Accession Number: BC010889
• Gene Symbol: CCDC53
• Gene ID (NCBI): 51019
• RRID: AB_2879551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; Architecture of the human interactome defines protein communities and disease networks.; Authors - Edward L Huttlin
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu