CCDC22 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCDC22 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 16636-1-AP

Synonyms

CCDC22, CXorf37, JM1

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Validation Data Gallery

• 

  WB analysis of human brain using 16636-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 16636-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 16636-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16636-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16636-1-AP (CCDC22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16636-1-AP

  IP result of anti-CCDC22 (IP:16636-1-AP, 4ug; Detection:16636-1-AP 1:600) with mouse lung tissue lysate 3600ug.

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Tested Applications

• Positive WB detected in: human brain tissue, mouse colon tissue, rat colon tissue, mouse lung tissue
• Positive IP detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

16636-1-AP targets CCDC22 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC22 fusion protein Ag9980
• Full Name: coiled-coil domain containing 22
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC000972
• Gene Symbol: CCDC22
• Gene ID (NCBI): 28952
• RRID: AB_2072065
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell; Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells.; Authors - Zharko Daniloski
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
mouse	WB	Nat Commun; CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.; Authors - Paulina Bartuzi
human	WB, IP	J Cell Biol; Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling.; Authors - Haiying Li
human	WB	Mol Metab; Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.; Authors - Liu Yang