CCDC158 Polyclonal antibody

CCDC158 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20396-1-AP

Synonyms

CCDC158

---

Validation Data Gallery

• 

  WB analysis of mouse testis using 20396-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20396-1-AP (CCDC158 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 20396-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20396-1-AP (CCDC158 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 20396-1-AP

  IP result of anti-CCDC158 (IP:20396-1-AP, 4ug; Detection:20396-1-AP 1:500) with mouse testis tissue lysate 6000ug.

• 

  IHC staining of human kidney using 20396-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 20396-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 20396-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 20396-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20396-1-AP targets CCDC158 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC158 fusion protein Ag14049
• Full Name: coiled-coil domain containing 158
• Calculated Molecular Weight: 1113 aa, 127 kDa
• Observed Molecular Weight: 127-140 kDa
• GenBank Accession Number: BC035224
• Gene Symbol: CCDC158
• Gene ID (NCBI): 339965
• RRID: AB_10694278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,IF	J Cell Physiol; CCDC158: A novel regulator in renal proximal tubular endocytosis unveiled through exome sequencing and interactome analysis; Authors - Tjessa Bondue