CCDC105 Polyclonal antibody

CCDC105 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 24026-1-AP

Synonyms

TEKTL1, Tektin-like protein 1, FLJ40365, Coiled-coil domain-containing protein 105

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Validation Data Gallery

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  WB analysis of DU 145 using 24026-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24026-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 24026-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24026-1-AP (CCDC105 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24026-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24026-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24026-1-AP (CCDC105 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse testis using 24026-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 24026-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CCDC105 antibody (24026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 24026-1-AP

  Immunofluorescent analysis of HeLa cells using 24026-1-AP (CCDC105 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, SH-SY5Y cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24026-1-AP targets CCDC105 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC105 fusion protein Ag21208
• Full Name: coiled-coil domain containing 105
• Calculated Molecular Weight: 499 aa, 57 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC035678
• Gene Symbol: CCDC105
• Gene ID (NCBI): 126402
• RRID: AB_2879406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.