c-Cbl Polyclonal antibody

• Featured Product
• KD/KO Validated

c-Cbl Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 25818-1-AP

Synonyms

CBL, EC:2.3.2.27, E3 ubiquitin-protein ligase CBL, CBL2, Casitas B-lineage lymphoma proto-oncogene

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Validation Data Gallery

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  WB analysis using 25818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 25818-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25818-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25818-1-AP (c-Cbl antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 25818-1-AP

  IP result of anti-c-Cbl (IP:25818-1-AP, 4ug; Detection:25818-1-AP 1:1000) with K-562 cells lysate 3680 ug.

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  IP experiment of K-562 using 25818-1-AP

  IP result of anti-c-Cbl (IP:25818-1-AP, 4ug; Detection:25818-1-AP 1:1000) with K-562 cells lysate 3680 ug.

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  IHC staining of human tonsillitis using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 25818-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25818-1-AP (c-Cbl antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, Raji cells, Ramos cells, Romas cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below

Product Information

25818-1-AP targets c-Cbl in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: c-Cbl fusion protein Ag23033
• Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Calculated Molecular Weight: 906 aa, 100 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC132733
• Gene Symbol: CBL
• Gene ID (NCBI): 867
• RRID: AB_2880252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).

Publications

Species	Application	Title
human	WB,IF	Hepatology; PMID: 29729186; Authors - Huixia Zhang ; KD Validated
human	WB,IF,CoIP	Cell Rep; Pro-prion, as a membrane adaptor protein for E3 ligase c-Cbl, facilitates the ubiquitination of IGF-1R, promoting melanoma metastasis; Authors - Huan Li ; KD Validated
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
human	WB	Cell Commun Signal; Gasdermin E regulates the stability and activation of EGFR in human non-small cell lung cancer cells; Authors - Limei Xu
mouse	WB	Front Med; Targeting deubiquitinase OTUB1 protects vascular smooth muscle cells in atherosclerosis by modulating PDGFRβ; Authors - Fei Xu
human	WB	Oncogene; AKIP1 accelerates glioblastoma progression through stabilizing EGFR expression; Authors - Sicheng Wan