Tested Applications
| Positive WB detected in | mouse heart tissue, human heart tissue, human skeletal muscle tissue, pig heart tissue, rat heart tissue |
| Positive IHC detected in | human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C2C12 cell |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18422-1-AP targets Calsequestrin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13246 Product name: Recombinant human CASQ2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-399 aa of BC022288 Sequence: MKRTHLFIVGIYFLSSCRAEEGLNFPTYDGKDRVVSLSEKNFKQVLKKYDLLCLYYHEPVSSDKVTQKQFQLKEIVLELVAQVLEHKAIGFVMVDAKKEAKLAKKLGFDEEGSLYILKGDRTIEFDGEFAADVLVEFLLDLIEDPVEIISSKLEVQAFERIEDYIKLIGFFKSEDSEYYKAFEEAAEHFQPYIKFFATFDKGVAKKLSLKMNEVDFYEPFMDEPIAIPNKPYTEEELVEFVKEHQRPTLRRLRPEEMFETWEDDLNGIHIVAFAEKSDPDGYEFLEILKQVARDNTDNPDLSILWIDPDDFPLLVAYWEKTFKIDLFRPQIGVVNVTDADSVWMEIPDDDDLPTAEELEDWIEDVLSGKINTEDDDEDDDDDDNSDEEDNDDSDDDDDE Predict reactive species |
| Full Name | calsequestrin 2 (cardiac muscle) |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC022288 |
| Gene Symbol | Calsequestrin 2 |
| Gene ID (NCBI) | 845 |
| RRID | AB_2071473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14958 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Calsequestrin 2 antibody 18422-1-AP | Download protocol |
| IHC protocol for Calsequestrin 2 antibody 18422-1-AP | Download protocol |
| WB protocol for Calsequestrin 2 antibody 18422-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Calsequestrin 2 Polyclonal antibody (Cat# 18422-1-AP) has 19 citations published across 17 journals, including Nature Chemical Biology, PNAS, EMBO Molecular Medicine, JCI Insight, Heart Rhythm, Stem Cell Reports, and Molecular Biology of the Cell. Research fields span cardiac physiology, arrhythmia mechanisms, calcium signaling, cardiomyopathy, muscle development, and metabolic regulation.
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Cardiac contraction and relaxation are regulated by distinct subcellular cAMP pools | ||
Proc Natl Acad Sci U S A Subcellular activation of β-adrenergic receptors using a spatially restricted antagonist | ||
Front Med Cardiomyocyte-specific long noncoding RNA Trdn-as induces mitochondrial calcium overload by promoting the m6A modification of calsequestrin 2 in diabetic cardiomyopathy | ||
EMBO Mol Med SK4 K(+) channels are therapeutic targets for the treatment of cardiac arrhythmias. | ||
NPJ Regen Med Leiomodin 2 neonatal dilated cardiomyopathy mutation results in altered actin gene signatures and cardiomyocyte dysfunction |
