CARM1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CARM1 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 55246-1-PBS

Synonyms

Protein arginine N-methyltransferase 4, PRMT4, Histone-arginine methyltransferase CARM1, EC:2.1.1.319, Coactivator-associated arginine methyltransferase 1

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Validation Data Gallery

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  IHC staining of human breast cancer using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55246-1-AP (CARM1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 55246-1-AP (CARM1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 55246-1-AP (same clone as 55246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55246-1-AP (CARM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 55246-1-AP (same clone as 55246-1-PBS)

  Immunofluorescent analysis of NIH/3T3 cells using 55246-1-AP (CARM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55246-1-PBS in a different storage buffer formulation.

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Product Information

55246-1-PBS targets CARM1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: coactivator-associated arginine methyltransferase 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 63-66 kDa, 55 kDa
• GenBank Accession Number: NM_199141
• Gene Symbol: CARM1
• Gene ID (NCBI): 10498
• RRID: AB_10863291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.