CAPZA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CAPZA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11806-1-AP

Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

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Validation Data Gallery

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  WB analysis of human brain using 11806-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11806-1-AP (CAPZA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 11806-1-AP

  IP result of anti-CAPZA1 (IP:11806-1-AP, 4ug; Detection:11806-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

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  IHC staining of human ovary tumor using 11806-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11806-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11806-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11806-1-AP (CAPZA1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human ovary tumor tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 5 publications below
• IP: See 1 publications below

Product Information

11806-1-AP targets CAPZA1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAPZA1 fusion protein Ag2267
• Full Name: capping protein (actin filament) muscle Z-line, alpha 1
• Calculated Molecular Weight: 286 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000144
• Gene Symbol: CAPZA1
• Gene ID (NCBI): 829
• RRID: AB_2070037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

Publications

Species	Application	Title
mouse	WB,IP	J Cell Biol; Heterodimeric capping protein is required for stereocilia length and width regulation.; Authors - Matthew R Avenarius
human	WB,IHC	Cell Mol Life Sci; HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.; Authors - Gemoll Timo T
human	WB	J Exp Clin Cancer Res; CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma.; Authors - Deng Huang ; KD Validated
human	IHC	Invest Ophthalmol Vis Sci; Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not.; Authors - Linge Annett A
human	WB,IHC	Int J Oncol; Prognostic value of CAPZA1 overexpression in gastric cancer.; Authors - Lee Young-Joon YJ ; KD Validated
human	WB,IHC	Melanoma Res; Differential expression patterns of capping protein, protein phosphatase 1, and casein kinase 1 may serve as diagnostic markers for malignant melanoma.; Authors - Sun Daxin D