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CAPRIN1 Polyclonal antibody
CAPRIN1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 15112-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse brain tissue, HEK-293T cells, rat brain tissue, HeLa cells, Jurkat cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, sodium arsenite treated HeLa cells, HepG2 cells, OC2 cells, cochlear cryosections|
|Positive FC detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
15112-1-AP targets CAPRIN1 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CAPRIN1 fusion protein Ag7099|
|Full Name||cell cycle associated protein 1|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||116 kDa|
|GenBank accession number||BC001731|
|Gene ID (NCBI)||4076|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.
|Product Specific Protocols|
|WB protocol for CAPRIN1 antibody 15112-1-AP||Download protocol|
|IHC protocol for CAPRIN1 antibody 15112-1-AP||Download protocol|
|IF protocol for CAPRIN1 antibody 15112-1-AP||Download protocol|
|IP protocol for CAPRIN1 antibody 15112-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lisa (Verified Customer) (02-20-2020)
The antibody works very well, giving specific bands in WB and a very good staining in IF.
Paul (Verified Customer) (04-30-2019)
Product works very well for WB, IP and IF using rat brain tissue samples.