CAPRIN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CAPRIN1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

73

Reviews (3)

Cat no : 15112-1-AP

Synonyms

GPI-anchored protein p137, GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1

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Validation Data Gallery

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  WB analysis using 15112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15112-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15112-1-AP (CAPRIN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15112-1-AP

  IP result of anti-CAPRIN1 (IP:15112-1-AP, 4ug; Detection:15112-1-AP 1:5000) with HeLa cells lysate 1600 ug.

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  IHC staining of human breast cancer using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 15112-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 15112-1-AP (CAPRIN1 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 15112-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15112-1-AP (CAPRIN1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of OC2 cells using 15112-1-AP

  IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with OC2 cells by Dr. Emily Towers.

• 

  IF Staining of HeLa using 15112-1-AP

  Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 15112-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAPRIN1 antibody (15112-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of cochlear cryosections using 15112-1-AP

  IF result of anti-CAPRIN1 (15112-1-AP, 1:200) with Cochlear cryosections by Dr. Emily Towers. CAPRIN1, purple; DAPI, blue.

• 

  FC experiment of NIH/3T3 using 15112-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human CAPRIN1 (15112-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, HEK-293T cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue, human testis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, sodium arsenite treated HeLa cells, HepG2 cells, OC2 cells, cochlear cryosections
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 53 publications below
• IHC: See 3 publications below
• IF: See 26 publications below
• IP: See 6 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Product Information

15112-1-AP targets CAPRIN1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, canine, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAPRIN1 fusion protein Ag7099
• Full Name: cell cycle associated protein 1
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC001731
• Gene Symbol: CAPRIN1
• Gene ID (NCBI): 4076
• RRID: AB_2070016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
human	WB	Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang ; KD Validated
human	WB,IF	Cell; Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.; Authors - David W Sanders
human	WB,IF	Nat Immunol; Inducible degradation of lncRNA Sros1 promotes IFN-γ-mediated activation of innate immune responses by stabilizing Stat1 mRNA.; Authors - Henan Xu
mouse	WB,RIP	Nat Commun; Fear extinction is regulated by the activity of long noncoding RNAs at the synapse; Authors - Wei-Siang Liau
	WB, IP	Neuron; CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation.; Authors - Silvia De Rubeis

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katherine (Verified Customer) (10-31-2023)

The antibody worked well with 1:5000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: 293T

FH

Lisa (Verified Customer) (02-20-2020)

The antibody works very well, giving specific bands in WB and a very good staining in IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: WB:1:2500 - IF:1:100
• Cell Tissue Type: Fibroblasts, lymphoblastoid cell line

CAPRIN1 Antibody Western Blot,Immunofluorescence, validation (WB:1:2500 - IF:1:100 dilution) in Fibroblasts, lymphoblastoid cell line (Cat no:15112-1-AP)

FH

Paul (Verified Customer) (04-30-2019)

Product works very well for WB, IP and IF using rat brain tissue samples.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Rat brain

CAPRIN1 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:2000 dilution) in Rat brain (Cat no:15112-1-AP)

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