CAPG-Specific Polyclonal antibody

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• KD/KO Validated

CAPG-Specific Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19535-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

• 

  WB analysis of mouse lung using 19535-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 19535-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse kidney tissue, U-937 cells
• Positive IHC detected in: human spleen tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19535-1-AP targets CAPG-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: capping protein (actin filament), gelsolin-like
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: NM_001747
• Gene Symbol: CAPG
• Gene ID (NCBI): 822
• RRID: AB_10638618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P40121
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Publications

What published studies show

The CAPG-Specific Polyclonal antibody (Cat# 19535-1-AP) has a total of 1 citation. It appears in Tohoku Journal of Experimental Medicine (2011), in a study investigating the association between gelsolin-like actin-capping protein overexpression and lung adenocarcinoma progression. The cited application was Western Blot in human samples. The research field falls under oncology, specifically lung cancer biology and tumor progression.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Tohoku J Exp Med; Overexpression of gelsolin-like actin-capping protein is associated with progression of lung adenocarcinoma.; Authors - Shao Fangchun F ; KD Validated