CAPG-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

CAPG-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 19535-1-AP

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

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Validation Data Gallery

• 

  WB analysis of mouse lung using 19535-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19535-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 19535-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19535-1-AP (CAPG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 19535-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19535-1-AP (CAPG-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse kidney tissue, U-937 cells
• Positive IHC detected in: human spleen tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

19535-1-AP targets CAPG-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: capping protein (actin filament), gelsolin-like
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: NM_001747
• Gene Symbol: CAPG
• Gene ID (NCBI): 822
• RRID: AB_10638618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Publications

Species	Application	Title
human	WB	Tohoku J Exp Med; Overexpression of gelsolin-like actin-capping protein is associated with progression of lung adenocarcinoma.; Authors - Shao Fangchun F ; KD Validated