CAMSAP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CAMSAP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

47

Cat no : 17880-1-AP

Synonyms

CAMSAP1L1, CAMSAP2, KIAA1078

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Validation Data Gallery

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  WB analysis using 17880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17880-1-AP (CAMSAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17880-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17880-1-AP (CAMSAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 17880-1-AP

  IP result of anti-CAMSAP2 (IP:17880-1-AP, 4ug; Detection:17880-1-AP 1:3000) with PC-3 cells lysate 5000ug.

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  IHC staining of human kidney using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human kidney tissue, human heart tissue, human skin tissue, human placenta tissue, human ovary tissue, human spleen tissue, mouse testis tissue, human testis tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 23 publications below
• IF: See 36 publications below
• CoIP: See 1 publications below

Product Information

17880-1-AP targets CAMSAP2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAMSAP2 fusion protein Ag11794
• Full Name: calmodulin regulated spectrin-associated protein 1-like 1
• Calculated Molecular Weight: 1478 aa, 167 kDa
• Observed Molecular Weight: 168 kDa
• GenBank Accession Number: BC065508
• Gene Symbol: CAMSAP2
• Gene ID (NCBI): 23271
• RRID: AB_2068826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAMSAP2, also known as CAMSAP1L1, is a microtubule minus-end binding protein, and is required for proper microtubule organization in neurons. CAMSAP2 stabilizes noncentrosomal microtubules and is important for neuronal polarity, axon specification, and dendritic branch formation.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC; Authors - Dipti Rai
human	IF	Nat Struct Mol Biol; A chemical genetics approach to examine the functions of AAA proteins.; Authors - Tommaso Cupido
human	IF	Nat Commun; Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner; Authors - Jielu Hao Robichaud
rat	WB,IF	Neuron; Microtubule Minus-End Binding Protein CAMSAP2 Controls Axon Specification and Dendrite Development.; Authors - Kah Wai Yau ; KD Validated
mouse	IF	Neuron; Katanin p80 regulates human cortical development by limiting centriole and cilia number.; Authors - Wen F Hu
rat	IF	Neuron; TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays.; Authors - Sam F B van Beuningen