CAMKMT Polyclonal antibody

CAMKMT Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat, Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27970-1-AP

Synonyms

C2orf34, CaM KMT, CAMKMT, CLNMT, FLJ23451

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Validation Data Gallery

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  WB analysis using 27970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27970-1-AP (CAMKMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 27970-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CAMKMT antibody (27970-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27970-1-AP targets CAMKMT in WB, IF/ICC, ELISA applications and shows reactivity with Mouse, Rat, Human samples.

• Tested Reactivity: Mouse, Rat, Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAMKMT fusion protein Ag27559
• Full Name: chromosome 2 open reading frame 34
• Calculated Molecular Weight: 323 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC053733
• Gene Symbol: CAMKMT
• Gene ID (NCBI): 79823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAMKMT, also named as C2orf34 and CLNMT, belongs to the class I-like SAM-binding methyltransferase superfamily and CLNMT methyltransferase family. The CAMKMT enzyme participates in cell cycle and development, regulating the production of calmodulin with three methyl lysine residues directly or indirectly. It also plays a major role in the calcium-dependent signaling pathway and posttranscriptional modifications, including Lys-115. CAMKMT is expressed mainly in the brain, liver, muscle, colon, and lung and is involved in muscle cell activities that are regulated by calcium binding (PMID: 27435482). The molecular weight of CAMKMT is 36 kDa.