CaMKII alpha Polyclonal antibody
CaMKII alpha Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13730-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 23 publications below |
IF | See 2 publications below |
Product Information
13730-1-AP targets CaMKII alpha in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, camel |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CaMKII alpha fusion protein Ag4745 |
Full Name | calcium/calmodulin-dependent protein kinase II alpha |
Calculated Molecular Weight | 478 aa, 54 kDa |
Observed Molecular Weight | 45-60 kDa |
GenBank Accession Number | BC040457 |
Gene Symbol | CaMKII alpha |
Gene ID (NCBI) | 815 |
RRID | AB_2070320 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of CAMK2 family.
Protocols
Product Specific Protocols | |
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WB protocol for CaMKII alpha antibody 13730-1-AP | Download protocol |
IHC protocol for CaMKII alpha antibody 13730-1-AP | Download protocol |
IF protocol for CaMKII alpha antibody 13730-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Lett MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma. | ||
Food Chem TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality. | ||
J Neuroinflammation Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model | ||
Chemosphere MCLR induces dysregulation of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in Sertoli cells. | ||
CNS Neurosci Ther miR-26a-5p alleviates CFA-induced chronic inflammatory hyperalgesia through Wnt5a/CaMKII/NFAT signaling in mice | ||
Exp Neurol MicroRNA-153 impairs hippocampal synaptic vesicle trafficking via downregulation of synapsin I in rats following chronic cerebral hypoperfusion. |