Calbindin-D28k Polyclonal antibody

• Featured Product
• KD/KO Validated

Calbindin-D28k Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Conjugate

Unconjugated

Publications

27

Reviews (4)

Cat no : 14479-1-AP

Synonyms

Calbindin, CALB1, D-28K, Calbindin D28, calbindin 1, 28kDa

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Validation Data Gallery

• 

  WB analysis using 14479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. (Negative control: NIH/3T3, C6)

• 

  WB analysis using 14479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14479-1-AP

  IP result of anti-Calbindin-D28k (IP:14479-1-AP, 4ug; Detection:14479-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of hippocampus using 14479-1-AP

  IHC result of Calbindin antibody (14479-1-AP, 1:5000 in 1% BSA 0.3%Tx-100 Tris buffer incubated at 4℃ overnight) with freshly formalin fixed human hippocampus (floating section). Secondary antibody biotinylated anti-rabbit lgG 1:200 in 1% BSA 0.3%Tx-100 Tris buffer for 2 hours at room temperature. .

• 

  IHC staining of human cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of rat cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14479-1-AP (Calbindin-D28k antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 14479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), PAX6 antibody (67529-1-Ig, Clone: 2C5A1, red).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), PAX6 antibody (67529-1-Ig, Clone: 2C5A1, red).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).

• 

  IF Staining of human kidney using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 14479-1-AP (Calbindin-D28k antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum tissue using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:1500 and Alexa Fluor 594 Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University)

• 

  IF Staining of mouse kidney using 14479-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using Calbindin-D28k antibody (14479-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human cerebellum tissue, hippocampus, human brain tissue, mouse cerebellum tissue, rat cerebellum tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue, human kidney tissue
• Positive IF-Fro detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 8 publications below
• IF: See 18 publications below

Product Information

14479-1-AP targets Calbindin-D28k in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calbindin-D28k fusion protein Ag5861
• Full Name: calbindin 1, 28kDa
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC006478
• Gene Symbol: Calbindin
• Gene ID (NCBI): 793
• RRID: AB_2228318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
mouse	IF	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	IF	Cell Death Differ; The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum.; Authors - Fei Wang
rat	IF	Autophagy; Autophagy and post-ischemic conditioning in retinal ischemia.; Authors - Biji Mathew
mouse	IHC,IF	Mol Ther; sEVsRVG selectively delivers antiviral siRNA to fetus brain, inhibits ZIKV infection and mitigates ZIKV-induced microcephaly in mouse model.; Authors - Rui Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Georgiana (Verified Customer) (09-11-2023)

Antibody labelled cell bodies and dendrites very clearly

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain

Calbindin-D28k Antibody Immunohistochemistry validation (1:1000 dilution) in mouse brain (Cat no:14479-1-AP)

FH

Ramzi (Verified Customer) (06-14-2023)

The brain vibratome sections were treated with Acid-Alcohol before the blocking solution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: brain tissue ''Vibratome ''

Calbindin-D28k Antibody Immunofluorescence validation (1:300 dilution) in brain tissue ''Vibratome '' (Cat no:14479-1-AP)

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

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