CACNA2D2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CACNA2D2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22145-1-AP

Synonyms

CACNA2D, CACNA2D2, KIAA0558, LUAC11.1

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 22145-1-AP

  WB result of CACNA2D2 antibody (22145-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CACNA2D2 transfected SH-SY5Y cells.

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  WB analysis using 22145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22145-1-AP (CACNA2D2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22145-1-AP targets CACNA2D2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACNA2D2 fusion protein Ag17580
• Full Name: calcium channel, voltage-dependent, alpha 2/delta subunit 2
• Calculated Molecular Weight: 1145 aa, 129 kDa
• Observed Molecular Weight: 100-112 kDa
• GenBank Accession Number: BC152438
• Gene Symbol: CACNA2D2
• Gene ID (NCBI): 9254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CACNA2D2 (calcium voltage-gated channel auxiliary subunit alpha2delta 2), also known as CASVDD. It is expected to be located in cell membrane. The protein is predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level). Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain (PMID: 10766861). The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel (PMID: 15111129). The molecular weight of CACNA2D2 is 129 kDa, and it can recognize other isoforms, which can be cut into fragments.