CA9 Polyclonal antibody

• Featured Product
• KD/KO Validated

CA9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 11443-1-AP

Synonyms

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 11443-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11443-1-AP (CA IX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11443-1-AP

  WB result of CA9 antibody (11443-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA9 transfected A549 cells.

• 

  IHC staining of human stomach cancer using 11443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11443-1-AP (CA9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11443-1-AP (CA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11443-1-AP targets CA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA9 fusion protein Ag1963
• Full Name: carbonic anhydrase IX
• Calculated Molecular Weight: 459 aa, 50 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC014950
• Gene Symbol: CA9
• Gene ID (NCBI): 768
• RRID: AB_2243631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Emma (Verified Customer) (10-28-2022)

Western blot, 30 µg lysate per well. Glioblastoma stem-like cells were cultured as small speroids and treated with CoCl2 to stabilise HIF-1a, or as medium or XL speroids (no CoCl2 treatment). There were are a few bands visible on the blot, but CoCl2 induced a band at around 60 kDa, and XL speroids which have a hypoxic core also resulted in a heavy band at the same height. See also: https://www.nature.com/articles/s41389-022-00433-3#Sec18 Figure S5 and supplementary methods for WB details.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma stem-like cells

CA9 Antibody Western Blot validation (1:1000 dilution) in Glioblastoma stem-like cells (Cat no:11443-1-AP)