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CA9 Polyclonal antibody

CA9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 11443-1-AP


CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

Tested Applications

Positive WB detected inmouse brain tissue, A549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11443-1-AP targets CA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA9 fusion protein Ag1963
Full Name carbonic anhydrase IX
Calculated Molecular Weight 459 aa, 50 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC014950
Gene Symbol CA9
Gene ID (NCBI) 768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.


Product Specific Protocols
WB protocol for CA9 antibody 11443-1-APDownload protocol
IHC protocol for CA9 antibody 11443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Emma (Verified Customer) (10-28-2022)

Western blot, 30 µg lysate per well. Glioblastoma stem-like cells were cultured as small speroids and treated with CoCl2 to stabilise HIF-1a, or as medium or XL speroids (no CoCl2 treatment). There were are a few bands visible on the blot, but CoCl2 induced a band at around 60 kDa, and XL speroids which have a hypoxic core also resulted in a heavy band at the same height. See also: https://www.nature.com/articles/s41389-022-00433-3#Sec18 Figure S5 and supplementary methods for WB details.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Glioblastoma stem-like cells
CA9 Antibody Western Blot validation (1:1000 dilution) in Glioblastoma stem-like cells (Cat no:11443-1-AP)