|Positive WB detected in||HL-60 cells, THP-1 cells|
|Positive IHC detected in||human colon cancer tissue, mouse ovary tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26416-1-AP is cGAS Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||cGAS fusion protein Ag23455|
|Full Name||chromosome 6 open reading frame 150|
|Calculated molecular weight||496 aa, 54 kDa|
|Observed molecular weight||58 kDa|
|GenBank accession number||BC113608|
|Gene ID (NCBI)||115004|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.
Immun Inflamm Dis
Activation of the cGAS-STING signaling pathway in adenomyosis patients.
Exp Eye Res
The cGAS-STING signaling pathway contributes to the inflammatory response and autophagy in Aspergillus fumigatus keratitis.
IFI16 regulates HTLV-1 replication through promoting HTLV-1 RTI-induced innate immune responses.
Small molecule inhibition of cyclic GMP-AMP synthase ameliorates sepsis-induced cardiac dysfunction in mice.
mTOR Dysregulation by Vaccinia Virus F17 Controls Multiple Processes with Varying Roles in Infection.
Restriction of HCMV replication by ISG15, a host effector regulated by cGAS-STING dsDNA sensing.
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Coralie (Verified Customer) (10-20-2021)
Cytosolic staining of cGAS (forms dots, after PFA fixation).