|Positive WB detected in||HL-60 cells, THP-1 cells|
|Positive IHC detected in||human colon cancer tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
26416-1-AP targets cGAS in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||cGAS fusion protein Ag23455|
|Full Name||chromosome 6 open reading frame 150|
|Calculated molecular weight||496 aa, 54 kDa|
|Observed molecular weight||58 kDa|
|GenBank accession number||BC113608|
|Gene ID (NCBI)||115004|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.
Signal Transduct Target Ther
TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion
Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit.
Adv Sci (Weinh)
PICH Supports Embryonic Hematopoiesis by Suppressing a cGAS-STING-Mediated Interferon Response.
J Clin Invest
Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration.
STING contributes to trauma-induced heterotopic ossification through NLRP3-dependent macrophage pyroptosis
Interactome and Proteome Dynamics Uncover Immune Modulatory Associations of the Pathogen Sensing Factor cGAS.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sonia (Verified Customer) (09-12-2023)
Very clear staining of ruptured micronuclei in U2OS cells, fixed in 4% FA and permeabilised with PBS-Triton 0.5%. Primary antibody incubation was performed o/n at 4ºC in blocking buffer at 1:250.
Shunbin (Verified Customer) (06-15-2023)
Produces a specific band and two other non-specific bands under the indicated conditions. The other cGAS Ab from Cell Signaling Technology produces one single band. (compare images attached
Zhiping (Verified Customer) (03-18-2022)
Good Ab for WB
Coralie (Verified Customer) (10-20-2021)
Cytosolic staining of cGAS (forms dots, after PFA fixation).