cGAS Polyclonal antibody

• Featured Product
• KD/KO Validated

cGAS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

93

Reviews (5)

Cat no : 26416-1-AP

Synonyms

Mab-21 domain-containing protein 1, h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150

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Validation Data Gallery

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  WB analysis using 26416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26416-1-AP (cGAS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26416-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (26416-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HL-60 cells, THP-1 cells
• Positive IHC detected in: human colon cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 75 publications below
• IHC: See 14 publications below
• IF: See 28 publications below
• IP: See 3 publications below

Product Information

26416-1-AP targets cGAS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, pig, bovine, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: cGAS fusion protein Ag23455
• Full Name: chromosome 6 open reading frame 150
• Calculated Molecular Weight: 496 aa, 54 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC113608
• Gene Symbol: cGAS
• Gene ID (NCBI): 115004
• RRID: AB_2880507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	WB,IF	Cell; Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit.; Authors - Nathan Meade
	WB,IF	Adv Sci (Weinh); PICH Supports Embryonic Hematopoiesis by Suppressing a cGAS-STING-Mediated Interferon Response.; Authors - Xinwei Geng
human	WB	Nat Commun; ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling; Authors - Maximilian Hirschenberger
	WB	Mol Cell; CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity; Authors - Guiheng Zhang ; KO Validated
rat	WB,IF	J Hazard Mater; GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline; Authors - Li Mou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hannah (Verified Customer) (12-10-2024)

Detects authentic cGAS band ~58kDa by western blot that disappears upon knockdown, but also detects a non-specific band just above, ~60kDa, which is not ideal for figure generation and concerning for other applications such as IF.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1,000 1h room temp
• Cell Tissue Type: human fibroblasts

cGAS Antibody Western Blot validation (1/1,000 1h room temp dilution) in human fibroblasts (Cat no:26416-1-AP)

FH

Sonia (Verified Customer) (09-12-2023)

Very clear staining of ruptured micronuclei in U2OS cells, fixed in 4% FA and permeabilised with PBS-Triton 0.5%. Primary antibody incubation was performed o/n at 4ºC in blocking buffer at 1:250.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: U2OS cells

FH

Shunbin (Verified Customer) (06-15-2023)

Produces a specific band and two other non-specific bands under the indicated conditions. The other cGAS Ab from Cell Signaling Technology produces one single band. (compare images attached

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

cGAS Antibody Western Blot validation (1:1000 dilution) in (Cat no:26416-1-AP)

FH

Zhiping (Verified Customer) (03-18-2022)

Good Ab for WB

• Applications: Western Blot

FH

Coralie (Verified Customer) (10-20-2021)

Cytosolic staining of cGAS (forms dots, after PFA fixation).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: HFF

cGAS Antibody Immunofluorescence validation (1/100 dilution) in HFF (Cat no:26416-1-AP)