C2orf49 Polyclonal antibody

C2orf49 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18272-1-AP

Synonyms

ASW, Ashwin

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Validation Data Gallery

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  WB analysis using 18272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18272-1-AP (C2orf49 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18272-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18272-1-AP (C2orf49 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 18272-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 18272-1-AP (C2orf49 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, Jurkat cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18272-1-AP targets C2orf49 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C2orf49 fusion protein Ag12948
• Full Name: chromosome 2 open reading frame 49
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC001310
• Gene Symbol: C2orf49
• Gene ID (NCBI): 79074
• RRID: AB_2066525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.