ERLEC1 Polyclonal antibody

ERLEC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29773-1-AP

Synonyms

C2orf30, CL24936, CL25084, Endoplasmic reticulum lectin 1, ER lectin, ERLEC1, Erlectin, XTP3 B, XTP3TPB

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Validation Data Gallery

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  WB analysis using 29773-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29773-1-AP (ERLEC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 29773-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29773-1-AP (ERLEC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29773-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29773-1-AP (ERLEC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse colon tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29773-1-AP targets ERLEC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERLEC1 fusion protein Ag31135
• Full Name: chromosome 2 open reading frame 30
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: NM_015701
• Gene Symbol: ERLEC1
• Gene ID (NCBI): 27248
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.