GID8 Polyclonal antibody

GID8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 24479-1-AP

Synonyms

C20orf11, GID8, Protein C20orf11, TWA1

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Validation Data Gallery

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  WB analysis using 24479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 24479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24479-1-AP (GID8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24479-1-AP (GID8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, HeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue, SW480 cells, HepG2 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 24479-1-AP is GID8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GID8 fusion protein Ag19751
• Full Name: chromosome 20 open reading frame 11
• Calculated Molecular Weight: 228 aa, 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC032120
• Gene Symbol: GID8
• Gene ID (NCBI): 54994
• RRID: AB_2879562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	PLoS Genet; Role of Bicaudal C1 in renal gluconeogenesis and its novel interaction with the CTLH complex.; Authors - Lucia Carolina Leal-Esteban
human	WB	Sci Rep; Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.; Authors - Shimaa H A Soliman
human	WB	bioRxiv; Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth; Authors - Nelson V Simwela
human	WB,IHC,IF	Cancer Lett; YTHDF1 regulates GID8-mediated glutamine metabolism to promote colorectal cancer progression in a m6A-dependent manner; Authors - Yicun Han
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (09-27-2020)

GID8 was pulled down with MAEA-flag, so I could tell which band is GID8. But without IP, it would be difficult to tell which band is the GID8, because the on-target band signal is too weak, while off-target band signals are strong in HEK293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

GID8 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:24479-1-AP)