C1orf83 Recombinant antibody

C1orf83 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240678F1

Cat no : 83626-5-RR

Synonyms

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

---

Validation Data Gallery

• 

  WB analysis using 83626-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83626-5-RR (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 83626-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using C1orf83 antibody (83626-5-RR, Clone: 240678F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 83626-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83626-5-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83626-5-RR is C1orf83 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: C1orf83 fusion protein Ag15040
• Full Name: chromosome 1 open reading frame 83
• Calculated Molecular Weight: 208 aa, 23 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC035374
• Gene Symbol: C1orf83
• Gene ID (NCBI): 127428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).