C1orf77 Polyclonal antibody

C1orf77 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27478-1-AP

Synonyms

FOP, CHTOP, PP7704, Friend of PRMT1 protein

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Validation Data Gallery

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  WB analysis using 27478-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27478-1-AP (C1orf77 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 27478-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf77 antibody (27478-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27478-1-AP targets C1orf77 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf77 fusion protein Ag26580
• Full Name: chromosome 1 open reading frame 77
• Calculated Molecular Weight: 248 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC002733
• Gene Symbol: C1orf77
• Gene ID (NCBI): 26097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.