C1orf56 Polyclonal antibody

C1orf56 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25433-1-AP

Synonyms

AF1Q, C1orf56, MENT, MLLT11, RP11 316M1.9

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Validation Data Gallery

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  WB analysis of mouse testis using 25433-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25433-1-AP (C1orf56 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25433-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25433-1-AP (C1orf56 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 25433-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25433-1-AP (C1orf56 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25433-1-AP is C1orf56 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf56 fusion protein Ag13860
• Full Name: chromosome 1 open reading frame 56
• Calculated Molecular Weight: 341 aa, 37 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC002469
• Gene Symbol: C1orf56
• Gene ID (NCBI): 54964
• RRID: AB_2880077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1orf56, also named as MENT, AF1Q and MLLT11, is involved in control of cellular proliferation. It is extreme expressed in several cancers especially in neuroblastoma. We detected the endogenous protein 35 kDa in mouse testis. We also get a 65 kDa which is unknown.

Publications

Species	Application	Title
human	WB	Front Mol Biosci; Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes.; Authors - Chenghao Zhanghuang