C1orf113 Polyclonal antibody

C1orf113 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25767-1-AP

Synonyms

C1orf113, cDNA FLJ58582, Homo sapiens (Human), SH3D21

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Validation Data Gallery

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  WB analysis of mouse testis using 25767-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25767-1-AP (C1orf113 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 25767-1-AP

  IP result of anti-C1orf113 (IP:25767-1-AP, 4ug; Detection:25767-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of mouse testis using 25767-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25767-1-AP (C1orf113 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 25767-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25767-1-AP (C1orf113 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25767-1-AP is C1orf113 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf113 fusion protein Ag22643
• Full Name: chromosome 1 open reading frame 113
• Calculated Molecular Weight: 640 aa, 71 kDa
• Observed Molecular Weight: 70-71 kDa
• GenBank Accession Number: BC101676
• Gene Symbol: C1orf113
• Gene ID (NCBI): 79729
• RRID: AB_2880232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.