C19orf70 Polyclonal antibody

• Featured Product
• KD/KO Validated

C19orf70 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 25514-1-AP

Synonyms

C19orf70, MIC13, P117, Protein P117, Protein QIL1, QIL1

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Validation Data Gallery

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  WB analysis using 25514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25514-1-AP (C19orf70 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25514-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25514-1-AP (C19orf70 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 25514-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25514-1-AP (C19orf70 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 25514-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25514-1-AP (C19orf70 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

The immunogen of 25514-1-AP is C19orf70 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C19orf70 fusion protein Ag22003
• Full Name: chromosome 19 open reading frame 70
• Calculated Molecular Weight: 118 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC042386
• Gene Symbol: C19orf70
• Gene ID (NCBI): 125988
• RRID: AB_2880112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C19orf70, also named as QIL1 and protein P117, belongs to the UPF0433 family. This antibody is specific to C19orf70.

Publications

Species	Application	Title
human	WB	EMBO J; Individual cristae within the same mitochondrion display different membrane potentials and are functionally independent.; Authors - Dane M Wolf
mouse	WB	iScience; Protease OMA1 modulates mitochondrial bioenergetics and ultrastructure through dynamic association with MICOS complex.; Authors - Martonio Ponte Viana
mouse	WB	Cytotherapy; Stem cells from human exfoliated deciduous teeth affect mitochondria and reverse cognitive decline in a senescence-accelerated mouse prone 8 model.; Authors - Wenhuan Guo
human	WB	mBio; Listeria monocytogenes Exploits Mitochondrial Contact Site and Cristae Organizing System Complex Subunit Mic10 To Promote Mitochondrial Fragmentation and Cellular Infection.; Authors - Filipe Carvalho ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zee (Verified Customer) (01-28-2020)

This antibody worked very well in adult mouse heart extracts when I performed western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue