C15orf53 Polyclonal antibody, PBS Only

C15orf53 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24553-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of A431 using 24553-1-AP (same clone as 24553-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 24553-1-AP (same clone as 24553-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 24553-1-AP (same clone as 24553-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24553-1-AP (C15orf53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 24553-1-AP (same clone as 24553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 24553-1-AP (same clone as 24553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 24553-1-AP (same clone as 24553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 24553-1-AP (same clone as 24553-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24553-1-AP (C15orf53 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24553-1-PBS in a different storage buffer formulation.

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Product Information

24553-1-PBS targets C15orf53 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C15orf53 fusion protein Ag18630
• Full Name: chromosome 15 open reading frame 53
• Calculated Molecular Weight: 179 aa, 20 kDa
• Observed Molecular Weight: ~30 kDa
• GenBank Accession Number: BC119016
• Gene Symbol: C15orf53
• Gene ID (NCBI): 400359
• RRID: AB_2879605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

C15orf53 has been identified on chromosome 15q14. It is probably neither causative for the etiology of BD (Bipolar disorder) nor for SCZD10 (periodic catatonia). Gene expression analysis revealed that C15orf53 was expressed in a subpopulation of leukocytes, but not in human post-mortem limbic brain tissue. In our detection this antibody always detect ~30 kDa band even the amino acids is 20 kDa.