C13orf18 Polyclonal antibody

C13orf18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21183-1-AP

Synonyms

C13orf18

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Validation Data Gallery

• 

  WB analysis of human skeletal muscle using 21183-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21183-1-AP (C13orf18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21183-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21183-1-AP (C13orf18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21183-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, MCF-7 cells
• Positive IHC detected in: human testis tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 21183-1-AP is C13orf18 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C13orf18 fusion protein Ag15474
• Full Name: chromosome 13 open reading frame 18
• Calculated Molecular Weight: 662 aa, 73 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC032311
• Gene Symbol: C13orf18
• Gene ID (NCBI): 80183
• RRID: AB_10733098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; Architecture of the human interactome defines protein communities and disease networks.; Authors - Edward L Huttlin
human	WB	Precis Clin Med; Blockade of Syk modulates neutrophil immune-responses via the mTOR/RUBCNL-dependent autophagy pathway to alleviate intestinal inflammation in ulcerative colitis; Authors - Fengqin Zhu