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Phospho-CD117/c-Kit (Tyr703) Recombinant antibody, PBS Only

Phospho-CD117/c-Kit (Tyr703) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G1

Cat no : 83925-1-PBS

Synonyms

CD117, KIT, EC:2.7.10.1, c-Kit, C Kit

Validation Data Gallery

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  • Western Blot (WB) analysis of Mo7e cells using Phospho-CD117/c-Kit (Tyr703) Recombinant antibody (83925-1-RR)

    WB analysis of Mo7e using 83925-1-RR (same clone as 83925-1-PBS)

    Non-treated Mo7e cells and SCF(HZ-1024) treated Mo7e cells were subjected to SDS PAGE followed by western blot with 83925-1-RR (Phospho-CD117/c-Kit (Tyr703) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83925-1-PBS in a different storage buffer formulation.

Product Information

83925-1-PBS targets Phospho-CD117/c-Kit (Tyr703) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Calculated Molecular Weight 976 aa, 110 kDa
Observed Molecular Weight140-160 kDa
GenBank Accession NumberBC071593
Gene Symbol CD117
Gene ID (NCBI) 3815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Kit is composed of five N-glycosylated immunoglobulin domains in the N-terminal extracellular portion that bind SCF, a transmembrane domain, and the carboxy-terminal intracellular tyrosine kinase domain. The binding of SCF autophosphorylates Kit on specific tyrosine residues (Tyr), such as Tyr568/570, Tyr703, Tyr721, and Tyr936. Kit then binds to other cytoplasmic enzymes, such as PI3K and Src kinases, and this complex activates downstream molecules. This activates the Akt-Bad pathway and the Ras-Mek-Erk cascade, which regulate gene expression and cytoskeletal structures, resulting in cell proliferation and survival. (PMID: 28403213)