Broad Range Prestained Protein Marker

Molecular Weight

3-245 kDa

Detection Method

Colorimetric

Number of Markers

13

Stain Type

3 colors: Blue, Red, Green

Cat no : PL00002


"Broad Range Prestained Protein Marker" Comparison

View side-by-side comparison of Broad Range Prestained Protein Marker from other vendors to find the one that best suits your research needs.
  • 4ul protein ladders were loaded in the gel with concentration 8%-18%. After Electrophoresis, the protein on the gel were transformed into the PVDF membrane. This image was taken by the iBright.

    4ul protein ladders were loaded in the gel with concentration 8%-18%. After Electrophoresis, the protein on the gel were transformed into the PVDF membrane. This image was taken by the iBright.

  • <strong>Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions</strong><br>The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.

    Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions
    The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.

  • Resolution of the broad range prestained protein ladder in a 4-12% Bis-Tris gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

    Resolution of the broad range prestained protein ladder in a 4-12% Bis-Tris gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.


Product Information

The PL00002 Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 prestained proteins covering a wide range of molecular weights from 3 to 245 kDa in Tris-Glycine buffer. The PL00002 Broad Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins.

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store product at 4 for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.

Usage

The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Detection Method

Colorimetric

Molecular Weight

~ 3, 10, 15, 20, 25, 35, 45, 60, 75, 100, 140, 180, 245 kDa

Number of Markers

13

Size Range

3 to 245

Stain Type

3 colors: Blue, Red, Green

Cited in Article as

PL00002, Broad Range Prestained Protein Marker, Proteintech, IL, USA

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christine (Verified Customer) (09-19-2023)

Very good protein and economical marker, same as PL00001 but with a few extra bands. 1 ul loaded on a SDS PAGE gel and transferred to nitrocellulose are enough for a clear detection of all the sizes. Of note: the pink marker (75 kDa) is not detected in the LICOR IR680 or IR800 channels but all others are OK.

  • Applications: Western Blot
FH

Andrea (Verified Customer) (12-03-2022)

A good, strong signal is provided by loading a few microliters of the product.

  • Applications: Western Blot
FH

Dan (Verified Customer) (09-30-2022)

Low price but you get a very good quality of protein ladder!

  • Applications: Western Blot,Protein purification
FH

Mary (Verified Customer) (09-19-2022)

Worked okay. It's a decent protein marker.

  • Applications: Western Blot
FH

Cloure (Verified Customer) (08-25-2022)

Worked pretty decently

  • Applications: Western Blot
  • Cell Tissue Type: Testicle
FH

Mickael (Verified Customer) (08-15-2022)

Worked like a charm!

  • Applications: Western Blot
FH

Maryna (Verified Customer) (08-13-2022)

Was very happy with the results, will ask my PI for to change

  • Applications: Western Blot
FH

Francisco (Verified Customer) (08-13-2022)

Performed very well in a pilot gel!

  • Applications: Western Blot
  • Cell Tissue Type: Spleen
FH

Miguel (Verified Customer) (08-09-2022)

Very nice, will be switching when starting new project

  • Applications: Western Blot
FH

Miguel (Verified Customer) (08-05-2022)

It worked great.

  • Applications: Western Blot
FH

Duncan (Verified Customer) (10-20-2021)

Perfect for all my Western blotting

  • Applications: Western Blot
FH

Adrian (Verified Customer) (09-02-2021)

Protein ladder we obtained from colleagues from Biochemistry worked great and exhibited clear, distinguishable bands. Good overall quality for research on food allergenicity. Would highly recommend.

  • Applications: Western Blot
  • Cell Tissue Type: N/A
FH

Maria (Verified Customer) (08-23-2021)

Cell lysate 10ug labelled with No-stain (590 nm emission). 4-15% TGX gel. PL00002 5 uL on the left. Very good marker.

  • Applications: Western Blot
FH

Phoebe (Verified Customer) (03-10-2020)

This ladder worked brilliantly on my western. I have a large protein so need a broader-range ladder, for which I have previously been using PageRuler Plus. The new Proteintech ladder has been a very good replacement.

  • Applications: Western Blot,