Beta galactosidase Recombinant antibody

Beta galactosidase Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240788F1

Cat no : 83706-6-RR

Synonyms

GLB1, ELNR1, EC:3.2.1.23, EBP, Beta-galactosidase

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Validation Data Gallery

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  WB analysis using 83706-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83706-6-RR (Beta galactosidase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83706-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta galactosidase antibody (83706-6-RR, Clone: 240788F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of A549 using 83706-6-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Beta galactosidase Recombinant antibody (83706-6-RR, Clone:240788F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83706-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83706-6-RR against Human Beta galactosidase were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, SH-SY5Y cells, HEK-293 cells, K-562 cells
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83706-6-RR is Beta galactosidase Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Beta galactosidase fusion protein Ag7792
• Full Name: galactosidase, beta 1
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 62-67 kDa
• GenBank Accession Number: BC007493
• Gene Symbol: GLB1/Beta-galactosidase
• Gene ID (NCBI): 2720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.