- Featured Product
- KD/KO Validated
ATG14/Barkor (N-terminal) Polyclonal antibody
ATG14/Barkor (N-terminal) Polyclonal Antibody for IF, IHC, WB,ELISA
Cat no : 19491-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, canine, bovine|
|Host / Isotype||Rabbit / IgG|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||65-68 kDa|
|GenBank accession number||NM_014924|
|Gene ID (NCBI)||22863|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).
|Product Specific Protocols|
|WB protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP||Download protocol|
|IHC protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP||Download protocol|
|IF protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Signal Transduct Target Ther
Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy
Adv Sci (Weinh)
Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia
STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.
Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.
Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Charlotte (Verified Customer) (01-25-2018)
I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.