BUD31 Polyclonal antibody

• Featured Product
• KD/KO Validated

BUD31 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, RIP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11798-1-AP

Synonyms

BUD31, BUD31 homolog (S. cerevisiae), EDG 2, EDG2, fSAP17, G10, Protein BUD31 homolog, Protein EDG 2, Protein G10 homolog, YCR063W

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Validation Data Gallery

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  WB analysis of mouse brain using 11798-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11798-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11798-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11798-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11798-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11798-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of hESC using 11798-1-AP

  hESC cells were subjected to SDS PAGE followed by western blot with 11798-1-AP (BUD31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11798-1-AP

  IP result of anti-BUD31 (IP:11798-1-AP, 4ug; Detection:11798-1-AP 1:1500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human colon cancer using 11798-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11798-1-AP (BUD31 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, hESC cells, human brain tissue, Jurkat cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, Raji cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Product Information

11798-1-AP targets BUD31 in WB, IP, IF, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BUD31 fusion protein Ag2373
• Full Name: BUD31 homolog (S. cerevisiae)
• Calculated Molecular Weight: 144 aa, 17 kDa
• Observed Molecular Weight: 17-20 kDa
• GenBank Accession Number: BC022821
• Gene Symbol: BUD31
• Gene ID (NCBI): 8896
• RRID: AB_2274894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; The spliceosome is a therapeutic vulnerability in MYC-driven cancer.; Authors - Tiffany Y-T Hsu ; KD Validated
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
mouse	WB,RIP	Cell Death Differ; Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation; Authors - Junchao Qin ; KD Validated
human	IHC,IF,CoIP,RIP	Nat Commun; Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12; Authors - Zixiang Wang ; KD Validated