BRUNOL5 Polyclonal antibody

BRUNOL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13557-1-AP

Synonyms

CELF-5, CELF5, CELF 5, Bruno-like protein 5, BRUNOL 5

---

Validation Data Gallery

• 

  WB analysis using 13557-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13557-1-AP (BRUNOL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13557-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13557-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 13557-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13557-1-AP (BRUNOL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-87 MG using 13557-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using BRUNOL5 antibody (13557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: HeLa cells, U-251 cells
• Positive IHC detected in: mouse brain tissue, human gliomas tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13557-1-AP targets BRUNOL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRUNOL5 fusion protein Ag4416
• Full Name: bruno-like 5, RNA binding protein (Drosophila)
• Calculated Molecular Weight: 485 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC028101
• Gene Symbol: BRUNOL5
• Gene ID (NCBI): 60680
• RRID: AB_1211624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRUNOL5, also named CELF5, is a 485 amino acid protein, which belongs to the CELF/BRUNOL family. BRUNOL5 is expressed in the brain and localizes in the nucleus. BRUNOL5 is an RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. BRUNOL5 specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. BRUNOL5 exists in two isoforms with MV 52 and 45 kDa.