BRMS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BRMS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16096-1-AP

Synonyms

Breast cancer metastasis-suppressor 1

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 16096-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16096-1-AP (BRMS1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of MCF-7 using 16096-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16096-1-AP (BRMS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16096-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16096-1-AP (BRMS1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human brain using 16096-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16096-1-AP (BRMS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 16096-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16096-1-AP (BRMS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 16096-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 16096-1-AP (BRMS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Product Information

16096-1-AP targets BRMS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRMS1 fusion protein Ag8939
• Full Name: breast cancer metastasis suppressor 1
• Calculated Molecular Weight: 246 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC009834
• Gene Symbol: BRMS1
• Gene ID (NCBI): 25855
• RRID: AB_2878217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Breast cancer metastasis suppressor 1 (BRMS1) was originally identified as a metastasis suppressor gene in human breast cancer. BRMS1 has since been reported to suppress metastasis in a variety of cancer types such as melanoma, ovarian carcinoma, and non-small lung cancer.

Publications

Species	Application	Title
human	WB,IP	Oncol Rep; BRMS1 participates in regulating cell sensitivity to DNA interstrand crosslink damage by interacting with FANCI.; Authors - Jianming Dou ; KD Validated
human	WB	Oncol Res; miR-346 Promotes HCC Progression by Suppressing Breast Cancer Metastasis Suppressor 1 Expression; Authors - Zhixian Guo
human	WB	J Neurooncol; Exploring the prognostic value of BRMS1 + microglia based on single-cell anoikis regulator patterns in the immunologic microenvironment of GBM; Authors - Songyun Zhao