BRCA1 Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00425-2

  Sandwich ELISA standard curve of MP00425-2, Human BRCA1 Recombinant Matched Antibody Pair - PBS only. 83390-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag17946. 83390-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP00425-2

  The mean BRCA1 concentration was determined to be 6.24 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load, 5.16 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 5.29 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load.

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  Cytometric bead array standard curve of MP00425-2

  Cytometric bead array standard curve of MP00425-2, BRCA1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83390-4-PBS. Detection antibody: 83390-2-PBS. Standard: Ag17946. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83390-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83390-4-PBS against Human BRCA1 was performed. The affinity constant is 0.417 nM.

BRCA1 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83390-4-PBS

Clone No.240285A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

PSCP, IRIS, EC:2.3.2.27, BROVCA1, BRCC1

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Product Information

83390-4-PBS targets BRCA1 as part of a matched antibody pair:

MP00425-2: 83390-4-PBS capture and 83390-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag17946

  Product name: Recombinant human BRCA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1514-1863 aa of BC115037

  Sequence: SGSLQNRNYPSQEGLIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQGPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSIVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY

• Full Name: breast cancer 1, early onset
• Calculated Molecular Weight: 1863 aa, 208 kDa
• GenBank Accession Number: BC115037
• Gene Symbol: BRCA1
• Gene ID (NCBI): 672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P38398
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.