BORG5/CDC42EP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BORG5/CDC42EP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27904-1-AP

Synonyms

BORG5, CDC42EP1, MSE55, CEP1, Cdc42 effector protein 1

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Validation Data Gallery

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  WB analysis using 27904-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27904-1-AP (BORG5/CDC42EP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, K-562 cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

27904-1-AP targets BORG5/CDC42EP1 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BORG5/CDC42EP1 fusion protein Ag27396
• Full Name: CDC42 effector protein (Rho GTPase binding) 1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC009356
• Gene Symbol: CDC42EP1
• Gene ID (NCBI): 11135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC42EP1, also known as BORG5, is a CDC42 binding protein that mediates actin cytoskeleton reorganization at the plasma membrane. This protein is secreted and is primarily found in bone marrow. BORG5 was discovered as a 55 kDa protein, but the accepted reference protein is only 40.3 kDa.

Publications

Species	Application	Title
human,canine	WB,IF	J Cell Sci; Borg5/Cdc42EP1 restricts contractility and motility in epithelial MDCK cells; Authors - David Cohen ; KD Validated