BCR Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

BCR Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22585-1-PBS

Synonyms

Renal carcinoma antigen NY-REN-26, EC:2.7.11.1, breakpoint cluster region, BCR1, BCR 1

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Validation Data Gallery

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  WB analysis of Y79 using 22585-1-AP (same clone as 22585-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 22585-1-AP (same clone as 22585-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 22585-1-AP (same clone as 22585-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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  WB analysis of COS-7 using 22585-1-AP (same clone as 22585-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 22585-1-AP (same clone as 22585-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 22585-1-AP (same clone as 22585-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 22585-1-AP (same clone as 22585-1-PBS)

  IP result of anti-BCR (IP:22585-1-AP, 4ug; Detection:22585-1-AP 1:1000) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 22585-1-AP (same clone as 22585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 22585-1-AP (same clone as 22585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22585-1-AP (same clone as 22585-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BCR antibody (22585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22585-1-AP (same clone as 22585-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using BCR antibody 22585-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 22585-1-PBS in a different storage buffer formulation.

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Product Information

22585-1-PBS targets BCR in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, monkey samples.

• Tested Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCR fusion protein Ag18281
• Full Name: breakpoint cluster region
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC066122
• Gene Symbol: BCR
• Gene ID (NCBI): 613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.