Validation Data Gallery
|Positive WB detected in||HEK-293 cells, A549 cells, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human lymphoma tissue, mouse spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23959-1-AP is BCL3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BCL3 fusion protein Ag21040|
|Full Name||B-cell CLL/lymphoma 3|
|Calculated molecular weight||446 aa, 47 kDa|
|Observed molecular weight||48-60 kDa|
|GenBank accession number||BC064993|
|Gene ID (NCBI)||602|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
BCL3 (BCL3 Transcription Coactivator), as a Protein Coding gene, has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis. In the cytoplasm, BCL3 inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, BCL3 acts as transcriptional activator that promotes transcription of NF-kappa-B target genes(Uniprot).
|Product Specific Protocols|
|WB protocol for BCL3 antibody 23959-1-AP||Download protocol|
|IHC protocol for BCL3 antibody 23959-1-AP||Download protocol|
|IF protocol for BCL3 antibody 23959-1-AP||Download protocol|
|IP protocol for BCL3 antibody 23959-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Nucleic Acids Res
SPaRTAN, a computational framework for linking cell-surface receptors to transcriptional regulators.
Mol Cancer Ther
The Discovery of a Novel Antimetastatic Bcl3 Inhibitor.
Bcl-3 promotes multi-modal tumour cell migration via NF-κB1 mediated regulation of Cdc42.
J Biol Chem
The proto-oncogene Bcl3 induces immune checkpoint PD-L1 expression, mediating proliferation of ovarian cancer cells.
Int J Oncol
BCL‑3 promotes cyclooxygenase‑2/prostaglandin E2 signalling in colorectal cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (08-16-2023)
Worked well for WB and IF
Ewelina (Verified Customer) (07-03-2019)
This is the only antibody for Western Blot, that worked for me for the detection of endogenous BCL3 in human lymphoma cell lines. It works perfectly.