BCL2 Recombinant antibody, PBS Only

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  WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80313-1-RR (BCL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using BCL2 antibody (80313-1-RR, Clone: 3O5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

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  IF Staining of human appendicitis using 80313-1-RR (same clone as 80313-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using BCL2 antibody (80313-1-RR, Clone: 3O5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80313-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80313-1-RR against Human BCL2 were performed. The affinity constant is 5.06 nM.

BCL2 {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 80313-1-PBS

Clone No.3O5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

BCL-2, 3O5

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Product Information

80313-1-PBS targets BCL2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCL2 fusion protein Ag3508
• Full Name: B-cell CLL/lymphoma 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC027258
• Gene Symbol: BCL2
• Gene ID (NCBI): 596
• RRID: AB_2918886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10415
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).