BCDIN3D Recombinant antibody, PBS Only (Capture)

BCDIN3D Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240152A9

Cat no : 83309-1-PBS

Synonyms

RNA 5'-monophosphate methyltransferase, EC:2.1.1.-, BCDIN3 domain-containing protein, 240152A9

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Validation Data Gallery

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  WB analysis using 83309-1-RR (same clone as 83309-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83309-1-RR (BCDIN3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83309-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83309-1-RR (same clone as 83309-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BCDIN3D Recombinant antibody (83309-1-RR, Clone:240152A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83309-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00333-1

  Cytometric bead array standard curve of MP00333-1, BCDIN3D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83309-1-PBS. Detection antibody: 83309-3-PBS. Standard: Ag15613. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00333-3

  Cytometric bead array standard curve of MP00333-3, BCDIN3D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83309-1-PBS. Detection antibody: 83309-4-PBS. Standard: Ag15613. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83309-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83309-1-PBS against Human BCDIN3D was performed. The affinity constant is 3.04 nM.

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  Affinity and Kinetic Characterization of 83309-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83309-1-RR against Human BCDIN3D were performed. The affinity constant is 2.38 nM.

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Product Information

83309-1-PBS targets BCDIN3D as part of a matched antibody pair:

MP00333-1: 83309-1-PBS capture and 83309-3-PBS detection (validated in Cytometric bead array)

MP00333-3: 83309-1-PBS capture and 83309-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCDIN3D fusion protein Ag15613
• Full Name: BCDIN3 domain containing
• Calculated Molecular Weight: 292 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC053560
• Gene Symbol: BCDIN3D
• Gene ID (NCBI): 144233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BCDIN3D (BCDIN3 domain containing RNA methyltransferase), it is expected to be located in cytoplasm, which is ubiquitous expressed in prostate, lymph node and skeletal muscle. This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. The molecular weight of BCDIN3D is 33 kDa.