AuthentiKine™ Human VEGF ELISA Kit
Cat no : KE00216
L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
KE00216 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous VEGF. The assay recognizes human VEGF. An antibody specific for VEGF has been pre-coated onto the microwells. The VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for VEGF is added to detect the captured VEGF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
|AuthentiKine™ Human VEGF ELISA Kit
|1 X 96 well plate
|Serum, Plasma, Cell culture supernatants
|Gene ID (NCBI)
|Cell culture supernatants
|mean ( pg/mL)
|mean ( pg/mL)
Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.
|All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
|L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
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