Atox1 Polyclonal antibody

Atox1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31090-1-AP

Synonyms

HAH1, Atx1

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Validation Data Gallery

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  WB analysis using 31090-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 31090-1-AP (Atox1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 31090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31090-1-AP (Atox1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NCI-H1299 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31090-1-AP targets Atox1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Atox1 fusion protein Ag35290
• Full Name: ATX1 (antioxidant protein 1) homolog 1 (yeast)
• Calculated Molecular Weight: 7 kDa
• Observed Molecular Weight: 7-10 kDa
• GenBank Accession Number: NM_009720
• Gene Symbol: Atox1
• Gene ID (NCBI): 11927
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.