Annexin V Monoclonal antibody

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• KD/KO Validated

Annexin V Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1E6A8

Cat no : 66245-1-Ig

Synonyms

Annexin A5, ANXA5, ANX5, Annexin-5, Annexin 5

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Validation Data Gallery

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  WB analysis using 66245-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66245-1-Ig

  WB result of Annexin V antibody (66245-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin V transfected HeLa cells.

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  WB analysis using 66245-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66245-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human malignant melanoma using 66245-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 66245-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human spleen using 66245-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 66245-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 66245-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 66245-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 66245-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (66245-1-Ig, Clone: 1E6A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of NIH/3T3 using 66245-1-Ig

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Annexin V Monoclonal antibody (66245-1-Ig, Clone:1E6A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, human urine exosomes tissue, Raw 264.7 cells, NIH/3T3 cells, K-562 cells, HepG2 cells
• Positive IHC detected in: human malignant melanoma tissue, human colon tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:15000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66245-1-Ig targets Annexin V in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin V fusion protein Ag1538
• Full Name: annexin A5
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC001429
• Gene Symbol: Annexin V
• Gene ID (NCBI): 308
• RRID: AB_2881634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Publications

Species	Application	Title
human	IF	PLoS Pathog; IL-17RA promotes pathologic epithelial inflammation in a mouse model of upper respiratory influenza infection; Authors - Zahrasadat Navaeiseddighi